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Table 1 In vivo readouts

From: Current challenges facing the assessment of the allergenic capacity of food allergens in animal models

Test Advantages Disadvantages
Gastrointestinal [54] Non-invasive, does not harm animals, qualitative and quantitative allergy scoring, blinded scoring possible No standardized scoring system, lab to lab variations, subjective, diarrhea as only GI specific sign
Systemic
 Anaphylaxis score [76] Non-invasive, qualitative and quantitative allergy scoring, blinded scoring possible Subjective, ethical consideration
Hypothermia [109, 110] Rectal temperature (semi-invasive), quantitative readout, blinded scoring possible Accuracy of rectal measurements, transplanted responders (invasive), ethical consideration
 Vascular leakage [24, 110] Qualitative readout, blinded scoring possible Invasive
Airways
 AHR [105] Qualitative and quantitative, objective readout, blinded measurements are possible, anesthesia not required for non-invasive AHR Invasive and anesthesia required (only for invasive AHR), usually endpoint measurement, expensive equipment required
Skin [111]
 PCA Quantitative measurement of skin thickness, qualitative assessement of vascular leakage Invasive, blinded scoring not possible
 ITH Quantitative measurement of skin swelling Invasive, blinded scoring not possible
 DTH Quantitative measurement of skin/tissue swelling Invasive, blinded scoring not possible