Table 1 In vivo readouts
Test | Advantages | Disadvantages |
|---|---|---|
Gastrointestinal [54] | Non-invasive, does not harm animals, qualitative and quantitative allergy scoring, blinded scoring possible | No standardized scoring system, lab to lab variations, subjective, diarrhea as only GI specific sign |
Systemic | ||
 Anaphylaxis score [76] | Non-invasive, qualitative and quantitative allergy scoring, blinded scoring possible | Subjective, ethical consideration |
Rectal temperature (semi-invasive), quantitative readout, blinded scoring possible | Accuracy of rectal measurements, transplanted responders (invasive), ethical consideration | |
Qualitative readout, blinded scoring possible | Invasive | |
Airways | ||
 AHR [105] | Qualitative and quantitative, objective readout, blinded measurements are possible, anesthesia not required for non-invasive AHR | Invasive and anesthesia required (only for invasive AHR), usually endpoint measurement, expensive equipment required |
Skin [111] | ||
 PCA | Quantitative measurement of skin thickness, qualitative assessement of vascular leakage | Invasive, blinded scoring not possible |
 ITH | Quantitative measurement of skin swelling | Invasive, blinded scoring not possible |
 DTH | Quantitative measurement of skin/tissue swelling | Invasive, blinded scoring not possible |