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Table 3 Diagnostic accuracy of the basophil activation test to peanut using different gating strategies

From: The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test

  Gating strategy
CD123+ HLADR− CD203c+ CD123+ HLADR−
Optimal cut-off 6.8 8.1
AUC ROC for the cut-off 0.91 0.97
Accuracy (%) 91 97
Sensitivity (%) 88 98
Specificity (%) 94 96
PPV (%) 93 95
NPV (%) 90 98
%True positives 40 45
%False positives 3 2
%True negatives 51 52
%False negatives 5 1
LR+ 14.7 24.4
LR− 0.13 0.02
  1. The optimal cut-off was determined for the %CD63+ basophils following stimulation with 100 ng/ml of peanut extract
  2. PPV positive predictive value, NPV negative predictive value, LR+ positive likelihood ratio, LR− negative likelihood ratio