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Table 2 Comparison of different strategies to gate on basophils

From: The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test

Parameters

Stimulants

CD123+ HLA-DR−

CD203c+

CD203c+ CD123+ HLA-DR−

Overall p value

p value1

p value2

p value3

Number of basophils

Negative controla

1722 (1226, 2184)

1782 (1334–2239)

1697 (1268–2134)

0.635

0.390

0.436

0.908

Anti-IgEb

1414 (972–1995)

2156 (1620–3097)

1939 (1452–2877)

<0.001

<0.001

0.06

<0.001

fMLPa

1572 (1037, 2100)

2146 (1684–2940)

1891 (1447–2715)

<0.001

<0.001

0.084

<0.001

Peanut extractc

1514 (914–2041)

2351 (1748–3062)

2104 (1615–2752)

<0.001

<0.001

0.348

0.001

%CD63 + basophils

Anti-IgEb

24.8 (10.7, 42.5)

29.4 (17.3–48.9)

32.0 (17.1–53.9)

0.021

0.020

0.809

0.013

fMLPa

28.2 (15.9, 42.3)

41.1 (26.3–53.4)

41.4 (26.9–56.9)

<0.001

<0.001

0.644

<0.001

Peanut extractc

32.3 (10.9, 56.2)

41.0 (18.0–56.9)

42.1 (20.0–68.5)

0.185

0.220

0.561

0.074

MFI CD63

Anti-IgEb

104.0 (26.3–195.2)

148 (36, 345)

159.3 (45.4–350.8)

0.019

0.029

0.611

0.009

fMLPa

139 (37, 284)

285 (76, 683)

307 (81, 708)

<0.001

<0.001

0.678

<0.001

Peanut extractc

138.4 (24.8–270.7)

190 (37, 513)

202.9 (42.6–567.4)

0.267

0.271

0.629

0.113

SI CD203c

Anti-IgEb

2.4 (1.5–3.8)

2.9 (2.0–4.2)

3.2 (2.0–4.6)

0.004

0.028

0.191

0.002

fMLPa

2.3 (1.7–3.1)

2.6 (2.2–3.7)

2.8 (2.2–4.1)

<0.001

0.001

0.280

<0.001

Peanut extractc

3.3 (1.8–5.3)

3.8 (2.4–4.8)

4.3 (2.4–5.4)

0.193

0.386

0.260

0.088

MFI CD203c

Anti-IgEb

2331 (197–5633)

4833 (254, 9020)

5013 (29–9161)

0.008

0.011

0.677

0.005

fMLPa

2763 (216–5139)

4549 (279, 7961)

4908 (334–8270)

0.001

0.002

0.813

0.001

Peanut extractc

3631.6 (179.4–8491.4)

5312 (206, 11,976)

7012 (236–11,857)

0.173

0.14

0.714

0.083

  1. Median and inter-quartile range are represented. Medians were compared between the three groups using Kruskall–Wallis test and between two groups using Mann–Whitney U test
  2. an = 116
  3. bn = 104 (non-responders were excluded)
  4. cn = 42 (peanut allergic)
  5. 1CD123+/HLA-DR− versus CD203c+
  6. 2CD203c+ versus CD203c+/CD123+/HLA-DR−
  7. 3CD123+/HLA-DR− versus CD203c+/CD123+/HLA-DR−
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Clinical and Translational Allergy

ISSN: 2045-7022

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