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Table 2 Comparison of different strategies to gate on basophils

From: The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test

Parameters Stimulants CD123+ HLA-DR− CD203c+ CD203c+ CD123+ HLA-DR− Overall p value p value1 p value2 p value3
Number of basophils Negative controla 1722 (1226, 2184) 1782 (1334–2239) 1697 (1268–2134) 0.635 0.390 0.436 0.908
Anti-IgEb 1414 (972–1995) 2156 (1620–3097) 1939 (1452–2877) <0.001 <0.001 0.06 <0.001
fMLPa 1572 (1037, 2100) 2146 (1684–2940) 1891 (1447–2715) <0.001 <0.001 0.084 <0.001
Peanut extractc 1514 (914–2041) 2351 (1748–3062) 2104 (1615–2752) <0.001 <0.001 0.348 0.001
%CD63 + basophils Anti-IgEb 24.8 (10.7, 42.5) 29.4 (17.3–48.9) 32.0 (17.1–53.9) 0.021 0.020 0.809 0.013
fMLPa 28.2 (15.9, 42.3) 41.1 (26.3–53.4) 41.4 (26.9–56.9) <0.001 <0.001 0.644 <0.001
Peanut extractc 32.3 (10.9, 56.2) 41.0 (18.0–56.9) 42.1 (20.0–68.5) 0.185 0.220 0.561 0.074
MFI CD63 Anti-IgEb 104.0 (26.3–195.2) 148 (36, 345) 159.3 (45.4–350.8) 0.019 0.029 0.611 0.009
fMLPa 139 (37, 284) 285 (76, 683) 307 (81, 708) <0.001 <0.001 0.678 <0.001
Peanut extractc 138.4 (24.8–270.7) 190 (37, 513) 202.9 (42.6–567.4) 0.267 0.271 0.629 0.113
SI CD203c Anti-IgEb 2.4 (1.5–3.8) 2.9 (2.0–4.2) 3.2 (2.0–4.6) 0.004 0.028 0.191 0.002
fMLPa 2.3 (1.7–3.1) 2.6 (2.2–3.7) 2.8 (2.2–4.1) <0.001 0.001 0.280 <0.001
Peanut extractc 3.3 (1.8–5.3) 3.8 (2.4–4.8) 4.3 (2.4–5.4) 0.193 0.386 0.260 0.088
MFI CD203c Anti-IgEb 2331 (197–5633) 4833 (254, 9020) 5013 (29–9161) 0.008 0.011 0.677 0.005
fMLPa 2763 (216–5139) 4549 (279, 7961) 4908 (334–8270) 0.001 0.002 0.813 0.001
Peanut extractc 3631.6 (179.4–8491.4) 5312 (206, 11,976) 7012 (236–11,857) 0.173 0.14 0.714 0.083
  1. Median and inter-quartile range are represented. Medians were compared between the three groups using Kruskall–Wallis test and between two groups using Mann–Whitney U test
  2. an = 116
  3. bn = 104 (non-responders were excluded)
  4. cn = 42 (peanut allergic)
  5. 1CD123+/HLA-DR− versus CD203c+
  6. 2CD203c+ versus CD203c+/CD123+/HLA-DR−
  7. 3CD123+/HLA-DR− versus CD203c+/CD123+/HLA-DR−