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Table 1 Number of basophils (gated as SSClow/CD123+/HLA-DR− cells) and expression of CD123 on the surface of basophils (gated as SSClow/CD203c+ cells) as measured by MFI of CD123-FITC in different stimulation conditions

From: The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test

Stimulant

n

Pre-stimulation

Post-stimulation

p value

Number of basophils

 fMLP

116

1722 (1226, 2184)

1572 (1037, 2100)

0.012

 Anti-IgE

104

1722 (1221, 2191)

1414 (972, 1995)

0.005

 Peanut extract

42

1732 (1212, 2174)

1514 (914, 2041)

0.134

MFI of CD123-FITC

 fMLP

116

1082 (98, 1549)

514 (75, 1020)

<0.001

 Anti-IgE

104

1081 (95, 1549)

495 (60, 1111)

<0.001

 Peanut extract

42

1172 (80–2058)

293 (40, 1244)

<0.001

  1. Median (inter-quartile range) is represented. p value refers to the comparison of post-stimulation conditions with the negative control