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Fig. 5 | Clinical and Translational Allergy

Fig. 5

From: The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test

Fig. 5

Gating with CD123/HLA-DR led to the loss-to-analysis of basophils and underestimation of basophil activation. In a representative experiment, basophils were gated as SSClow/CD123bright/HLA-DR− (a) or SSClow/CD203c+/CD123+/HLA-DR− (b). The percentage of CD63+ basophils is represented in different conditions: negative control, peanut extract 100 ng/ml and peanut-dose–response curve. Note the bi-exponential display [38]

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