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Fig. 2 | Clinical and Translational Allergy

Fig. 2

From: Assessment of sensitization to grape and wine allergens as possible causes of adverse reactions to wine: a pilot study

Fig. 2

ImmunoBlot of IgE antibody reactivity applying different grape and wine proteins. a ImmunoBlot of participant No. 7, who showed positive reactions in all tests; b Coomassie-stained 15 % SDS-PAGE. Samples: 1: peach; 2: red grapes; 3: Dornfelder grapes (winery Fleischer, Mainz); 4: white grapes; 5: Dornfelder wine (after PVP precipitation); 6: purified LTP from Dornfelder wine [15]; 7: purified LTP from Dornfelder grapes [15]; 8: Riesling wine (after Lyophilisation); 9: RunBlue Dual Color SDS marker (Expedeon, Cambridge,United Kingdom). For SDS-PAGE 30 μl of the extract, which was treated 3:1 with SDS sample buffer and incubated 95 °C for 10 min, was applied

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