Figure 4

Protein modifications induced by the denaturation process. Native PAGE for native (N) and denatured (D) BLG, ALA and β-casein (A) and CD spectra of BLG (B); ALA (C) and β-casein (D) in native (solid line) and denatured (dashed line) states. All spectra were recorded in PBS buffer at 20°C in a 1-mm quartz cuvette.