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Figure 2 | Clinical and Translational Allergy

Figure 2

From: Inability to detect significant absorption of immunoreactive soya protein in healthy adults may be relevant to its weak allergenicity

Figure 2

Detection of soya allergen in serum from soya challenged subjects. A) Serum samples (0, 1, 3 and 24 hour after ingestion of soya meal) were analyzed by ELISA. Results were corrected from background (0 hour serum sample) and samples with an OD > 0.023 ng/ml (mean + 3 x SD, 0 h samples) were considered positive for soya. Gray zone indicate cut off value. B) Human basophils (n = 2 donors) sensitized with IgE from soya allergic or healthy control were stimulated with serum from the challenged subjects (0, 1, 3 and 24 hour after ingestion of soya meal). Results (% released histamine,%HR) were corrected from background (0 hour serum sample) and samples with a%HR > 9.3% (mean + 3 x SD, control serum) were considered positive for soya. Gray zones indicate cut off value. White square: 1 hour, Gray square: 3 hour, Black square: 24 hour.

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