Figure 2From: Inability to detect significant absorption of immunoreactive soya protein in healthy adults may be relevant to its weak allergenicityDetection of soya allergen in serum from soya challenged subjects. A) Serum samples (0, 1, 3 and 24 hour after ingestion of soya meal) were analyzed by ELISA. Results were corrected from background (0 hour serum sample) and samples with an OD > 0.023 ng/ml (mean + 3 x SD, 0 h samples) were considered positive for soya. Gray zone indicate cut off value. B) Human basophils (n = 2 donors) sensitized with IgE from soya allergic or healthy control were stimulated with serum from the challenged subjects (0, 1, 3 and 24 hour after ingestion of soya meal). Results (% released histamine,%HR) were corrected from background (0 hour serum sample) and samples with a%HR > 9.3% (mean + 3 x SD, control serum) were considered positive for soya. Gray zones indicate cut off value. White square: 1 hour, Gray square: 3 hour, Black square: 24 hour.Back to article page