Production of cysLTs (LTC4/D4/E4) and TXB2 in response to arachidonic acid (AA) in vitro. Airway tissues from sensitized guinea pigs were stimulated in vitro with three different doses of AA. Arachidonic acid enhanced the production of cysLTs and TXB2 in airway tissue of animals exposed to either PBS or low dose TMA-GPSA on five consecutive days in a dose dependent manner (Spearman Rank Correlation test Rho = 0.9, p < 0.001 for cysLTs vs. doses of AA, Rho = 0.6, p < 0.03 for TXB2 vs. doses of AA). Repeated allergen exposure significantly enhanced the capacity of airway tissue to produce cysLTs and TXB2 in vitro compared to PBS-exposure. Data are expressed as mean ± SEM, Mann-Whitney U test, *p < 0.05, n = 8 in each group.