Figure 1

Characterization and validation of the rabbit anti-Sin a 2 serum. A) ELISA titration of Sin a 2 (0.2 μg/well) and mustard extract (20 μg/well) using the rabbit specific anti-Sin a 2 serum. B) Immunoblotting of mustard extract (50 μg/lane) or purified Sin a 2 (0.5 μg/lane) using different dilutions of the anti-Sin a 2 serum. Molecular mass markers are displayed in the left margin. C) ELISA-inhibition experiments of the IgG-binding to Sin a 2 or mustard extract-coated wells with increasing concentrations of Sin a 2 or mustard as inhibitors. D) Immunoblotting-inhibition experiments of the IgG-binding to nitrocellulose blotted mustard extract (50 μg/lane) or Sin a 2 (0.5 μg/lane) using as inhibitors BSA (20 μg/mL), Sin a 2 (20 μg/ml) or mustard (1 mg/ml). Molecular mass markers are displayed in the left margin.