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Figure 1 | Clinical and Translational Allergy

Figure 1

From: Effect of simulated gastro-duodenal digestion on the allergenic reactivity of beta-lactoglobulin

Figure 1

Characterization of blg digesta. A) phase 1 digestion: Lane 1 BLG @ 5 mg/ml, +PC (phosphatidyl choline) +E (enzyme). Lane 2 BLG @ 0.5 mg/ml, +PC +E. Lane 3 BLG @ 5 mg/ml, +PC -E. Lane 4 BLG @ 0.5 mg/ml, +PC -E. Lane 5 BLG @ 5 mg/ml, -PC +E. Lane 6 BLG @ 0.5 mg/ml, -PC +E. Lane 7 BLG @ 5 mg/ml, -PC -E. Lane 8 BLG @ 0.5 mg/ml, -PC -E. Lane 9 BLG @ 5 mg/ml. B) phase 1+2 digestion: Lane 1, BLG @ 5 mg/ml, +PC (phosphatidyl choline) +E (enzyme). Lane 2 BLG @ 0.5 mg/ml, +PC +E. Lane 3 BLG @ 5 mg/ml, +PC -E. Lane 4 BLG @ 0.5 mg/ml, +PC -E. Lane 5 BLG @ 5 mg/ml, -PC +E. Lane 6 BLG @ 0.5 mg/ml, -PC +E. Lane 7 BLG @ 5 mg/ml, -PC -E. Lane 8 BLG @ 0.5 mg/ml, -PC -E. Lane 9 BLG @ 5 mg/ml. Lane 10 BLG @ 0.5 mg/ml. Asterisk (*) denotes BLG SDS resistant dimmer, triangle (Δ) denotes Phosphatidyl choline.

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