Volume 4 Supplement 3
Analysis of B cell subsets in severe cutaneous adverse reaction
© Azukizawa et al; licensee BioMed Central Ltd. 2014
Published: 18 July 2014
T cells play an important role in the pathogenesis of severe cutaneous adverse reaction (SCAR), while the role of B cell immunity in SCAR is unclear. It has been reported that number of B cell and serum IgG are decreased in the patient's peripheral blood of drug-induced hypersensitivity syndrome (DIHS) / drug reaction with eosinophilia and systemic symptoms (DRESS) in the acute stage. Regulatory B cell (Breg) is a IL-10 producing cell and negatively regulates cellular immunity in the mouse autoimmune disease model. Also in the mouse model, B-1 B cell secretes immunoglobulin against microbial infection in a T cell-independent manner. Recently, Breg and B-1 B cell were identified as minor populations of human B cells in the peripheral blood, however, the roles of these B cells in SCAR are unknown. Here, we studied the ratio of Breg and B-1 B cells in the peripheral blood B cells of SCAR patients.
Three patients of toxic epidermal necrolysis (TEN), DIHS/DRESS, and three healthy controls were involved in this study. Peripheral blood mononuclear cells of SCAR patients in both acute stage and recovery stage were stained. CD24hi, CD27+, CD19+ cells and CD27+, CD43+, CD20+ cells were analyzed as Breg and B-1 B cells, respectively.
The ratio of B cells in the peripheral blood was decreased in the acute stage SCAR. Furthermore, the ratio of Breg in the CD19+ cells was decreased in the acute stage of DIHS. On the other hand, the ratio of B-1 B cells was different among patients of TEN and DIHS and there was no clear tendency.
Although the pathological roles of Breg and B-1 B cell in SCAR are still unclear, they might contribute to drug-specific T cell activation or herpes virus reactivation.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.