Volume 4 Supplement 2

5th International Symposium on Molecular Allergology (ISMA 2013)

Open Access

Colonisation of germ-free mice with probiotic lactobacilli mitigated allergic sensitisation in murine model of birch pollen allergy

  • Hana Kozakova1,
  • Martin Schwarzer2,
  • Dagmar Srutkova2,
  • Tomas Hudcovic2 and
  • Bozena Cukrowska3
Clinical and Translational Allergy20144(Suppl 2):P26

DOI: 10.1186/2045-7022-4-S2-P26

Published: 17 March 2014

Background

Allergies are becoming a serious health burden in developed countries. Increasing numbers of clinical trials and animal experiments show that probiotics are new promising tools in allergy prophylaxis. In the present work we compared anti-allergic properties of the recently described probiotic mixture of Lactobacillus casei LOCK0900, L. casei LOCK0908 and L. paracasei LOCK0919 and L. plantarum NCIMB8826 using gnotobiotic experimental model of birch pollen allergy.

Methods

Germ-free (GF) BALB/c mice were colonized with lactobacilli mixture or with L. plantarum. Colonized mice and age-matched GF controls were repeatedly immunized with Bet v 1. Allergen-specific antibody levels, total IgA and IgE, and transforming growth factor (TGF)-beta were determined in sera. Th1/Th2 cytokine response was measured in supernatants of spleen and mesenteric lymph node (MLN) cell cultures.

Results

Mice colonized with lactobacilli mixture showed significantly lower value of Bet v 1-specific IgG1, IgG2a and IgE and elevated levels of total IgA in sera and intestinal lavages and increase of TGF-beta compared to the GF sensitized group. We observed no such changes in L. plantarum colonized mice. Splenocytes of mice colonized with lactobacilli mixture, cultivated with Bet v 1, showed up-regulation of TGF-beta; in contrast in mice colonized with L. plantarum it was down-regulated. In MLN the colonization increased production of TGF-beta and reduced IL-5 in both groups, L. plantarum colonization increased IFN-gamma production.

Conclusion

Colonization with lactobacilli mixture inhibited the development of allergic immune responses through induction of regulatory cytokine TGF-beta and can be thus exploited for alleviation of pollen allergies. Supported by grants 303/09/0449 of the Czech Science Foundation, CZ.3.22/2.1.00/09.01574 and WTZ CZ16 of the OEAD, NR12-0101-10/2011 of the Republic of Poland.

Authors’ Affiliations

(1)
Institute of Microbiology of the Czech Academy of Sciences of the Czech Republic
(2)
Institute of Microbiology of the Czech Academy of Sciences of the Czech Republic, Laboratory of Gnotobiology
(3)
Department of Pathology, The Children’s Memorial Health Institute

Copyright

© Kozakova et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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