Volume 4 Supplement 2

5th International Symposium on Molecular Allergology (ISMA 2013)

Open Access

Analysis of the IgE- and IgG-reactivity profiles of asthmatic and non-asthmatic HDM-allergic patients using the ISAC microarray system

  • Yvonne Resch1,
  • Sven Michel2,
  • Michael Kabesch3,
  • Rudolf Valenta1 and
  • Susanne Vrtala4
Clinical and Translational Allergy20144(Suppl 2):O17

DOI: 10.1186/2045-7022-4-S2-O17

Published: 17 March 2014

Background

House dust mites (HDM) represent one of the most important inducers for respiratory allergies worldwide. The aim of this study was to investigate the IgE- and IgG-reactivity profiles of HDM-allergic individuals suffering only from allergic rhinitis or from allergic asthma.

Methods

This study included sera from clinically well characterized asthmatic (n=105) and non-asthmatic (n=53) HDM-allergic patients. IgE- and IgG-reactivity to seven HDM-allergens (nDer p 1, rDer p 2, rDer p 5, rDer p 7, rDer p 10, rDer p 21 and rDer p 23) were measured using a customized allergen microarray (i.e., ISAC chip, Thermofisher, Vienna, Austria).

Results

HDM-allergic individuals suffering from asthma showed striking differences regarding their IgE reactivity profiles compared to the non-asthmatic group. First, the frequency of IgE reactivity to the tested HDM allergens was up to 3-fold higher in the asthmatic than in the non-asthmatic group. Seventy percent of the asthmatics reacted with 3 to 6 of the tested allergens compared to only 45% in the non-asthmatic group. Furthermore, IgE-levels to nDer p 1, rDer p 2, rDer p 5 and rDer p 23 were significantly higher in the asthmatic group. In contrast, HDM-allergic asthma patients showed a lower IgG-binding frequency to the seven HDM allergens than HDM-allergic patients without asthma.

Conclusion

The IgE and IgG reactivity profiles to HDM allergens differ considerably in patients with mild (i.e., rhinitis) and severe (i.e., asthma) respiratory symptoms due to HDM allergy.

Authors’ Affiliations

(1)
Medical University of Vienna, Department of Pathophysiology and Allergy Research
(2)
Hannover Medical School, Dep. of Ped. Pneumology, Allergy and Neonatology
(3)
University Children`s Hospital Regensburg (KUNO), Department of Pediatric Pneumology and Allergy
(4)
Medical University of Vienna, CD Lab. for the Development of Allergen Chips

Copyright

© Resch et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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