Volume 1 Supplement 1

Food Allergy and Anaphylaxis Meeting 2011

Open Access

Cross-reactivity of pollen and food allergens: soybean Gly m 4 is a member of the Bet v 1 superfamily and closely resembles yellow lupine proteins

  • Paul Rösch1,
  • Hanna Berkner1,
  • Philipp Neudecker1,
  • Diana Mittag2,
  • Barbara Ballmer-Weber2,
  • Peter Lehn3,
  • Kristian Schweimer1 and
  • Stefan Vieths4
Clinical and Translational Allergy20111(Suppl 1):P6

DOI: 10.1186/2045-7022-1-S1-P6

Published: 12 August 2011

In many cases, patients allergic to birch pollen also show allergic reactions after ingestion of plant-derived food. This observation is explained on the molecular level by cross-reactivity of IgE antibodies induced by sensitization to the major birch pollen allergen Bet v 1 with homologous food allergens. As IgE antibodies recognize conformational epitopes, a precise structural characterization of the allergens involved is necessary to understand cross-reactivity and thus to develop new methods of allergen-specific immunotherapy for allergic patients. Here we present the three-dimensional solution structure of the soybean allergen Gly m 4, a member of the superfamily of Bet v 1 homologous proteins and a cross-reactant with IgE antibodies originally raised against Bet v 1, as shown by immunoblot inhibition and histamine release assays. Although the overall fold of Gly m 4 is very similar to that of Bet v 1, the three-dimensional structures differ in detail. The Gly m 4 local structures that display those differences are also found in proteins from yellow lupine. The three-dimensional structure of Gly m 4 in combination with immunological data allows us to propose surface patches that might represent cross-reactive epitopes.

Authors’ Affiliations

(1)
Universität Bayreuth, Biopolymere
(2)
University Hospital, Dermatology
(3)
Klinische Chemie und Laboratoriumsmedizin, Universitätsklinikum Regensburg
(4)
Paul-Ehrlich-Institut, Allergologie

Copyright

© Rösch et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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